| Gene name |
SPBC15D4.07c |
| Gene ID |
27/H08 |
| Gene synonyms/obsolete |
apg9 |
| Gene product |
involved in autophagy;
involved in cytoplasm to vacuole targeting |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2109 |
| ORF length (spliced) |
|
| Entry clone length |
2109 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
640T:C / 1169A:G /
1287T:C / 1998T:C |
| Comments |
ApaI or SacII should
be used instead of NotI for integration using pDUAL. |
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC15D4.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTTATCAGCCGGCTCA |
| Rev primer name |
SPBC15D4.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACGAAGATCCCTTCTAGGT |
| Amino acid length |
702 |
| Molecular weight |
81.7 |
| Isoelectric point (calc.) |
7.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
6 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWLFLSFLLAL/LGSFTAILVI |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal
|