| Gene name |
SPBC146.06c |
| Gene ID |
27/H10 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved hypothetical
protein; similar to O. sativa Q9LWI8 A. thaliana F11A17.9
human KIAA1018 and C. elegans C01G5.8; no Sc ortholog;
non-essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2112 |
| ORF length (spliced) |
|
| Entry clone length |
2112 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
858A:G / 1331T:C /
1620A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC146.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAACGATTCTTTAATGA |
| Rev primer name |
SPBC146.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTTTTTTGTGCATGGAG |
| Amino acid length |
703 |
| Molecular weight |
82.2 |
| Isoelectric point (calc.) |
8.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEYVEVLEL |
| Localization (YFP) |
nucleus; nuclear
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |