| Gene name |
SPAC20G4.03c |
| Gene ID |
28/A04 |
| Gene synonyms/obsolete |
hri1 |
| Gene product |
serine/threonine
protein kinase; eIF2 kinase; possibly regulates initiation of
translation by phosphorylation of eIF2alpha; similar to Sp
hri2 (paralog); no apparent Sc ortholog |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
2115 |
| ORF length (spliced) |
|
| Entry clone length |
2115 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC20G4.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTCGGCACTTCTACTAA |
| Rev primer name |
SPAC20G4.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACAGTCGTAATTGGGGTTT |
| Amino acid length |
704 |
| Molecular weight |
79 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |