Gene name |
SPAC3F10.01 |
Gene ID |
28/A08 |
Gene synonyms/obsolete |
nda4; mcm5;
SPAC1B2.05 |
Gene product |
MCM complex subunit;
AAA family ATPase; involved in DNA replication (initiation)
(required); ATP dependent; DNA helicase activity |
Entry clone |
Cloned |
ORF length (unspliced) |
2244 |
ORF length (spliced) |
2163 |
Entry clone length |
2244 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
1559A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3F10.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGCAGCAACAGGTTG |
Rev primer name |
SPAC3F10.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATACTCCTGATCGATACACC |
Amino acid length |
720 |
Molecular weight |
80 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRGDINVLLL |
Localization (YFP) |
cytosol; cytoplasmic
dots by over expression |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
Microscope used for
observation |
Leica |