| Gene name |
SPBC1706.03 |
| Gene ID |
28/B10 |
| Gene synonyms/obsolete |
SPBC839.01 |
| Gene product |
putative GTPase;
GTP-binding protein; possibly involved in mitochondrial
fusion; involved in mitochondrial DNA (maintenance) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2277 |
| ORF length (spliced) |
|
| Entry clone length |
2277 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1589C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1706.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGAAGAGTGCAAGGCA |
| Rev primer name |
SPBC1706.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATAATTTATATCACATCCC |
| Amino acid length |
758 |
| Molecular weight |
87.1 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSGLVGDILNI/LQKHFRFELGL/LQHLLVPVLGL |
| Localization (YFP) |
ambiguous
structure |
| Comments for localization |
ambiguous structure
near nucleus |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |