Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC887.03c
Gene ID 28/C04
Gene synonyms/obsolete
Gene product basic helix-loop-helix (bHLH); involved in DNA replication; NucleOlar Complex 2; involved in nuclear export of pre-ribosomes; interacts with MCM proteins; involved in the establishment and maintenance of pre-replication complexes; conserved eukaryotic protein
Entry clone Cloned
ORF length (unspliced) 2289
ORF length (spliced) 2244
Entry clone length 2289
No. of intron 1
Sequence status Finished
Sequence results 648A:G / 1686A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC887.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCGGCTCGGAAGAATCA
Rev primer name SPBC887.03.Rv
Rev primer SEQ AGAAAGCTGGGTACAACGAAGTGCTTTTTAAC
Amino acid length 747
Molecular weight 85.1
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 442
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LEEPVENLHL/LPDVYDLFL/LSSFSKRLAI
Localization (YFP) nucleolus>>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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