Gene name |
SPBC1703.04 |
Gene ID |
28/C06 |
Gene synonyms/obsolete |
mlh1 |
Gene product |
mutL homolog; involved
in DNA repair; involved in mismatch repair |
Entry clone |
Cloned |
ORF length (unspliced) |
2295 |
ORF length (spliced) |
2055 |
Entry clone length |
2295 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
1760T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1703.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGTCAATTCACGAGC |
Rev primer name |
SPBC1703.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACAACGTTCGAAAACATTA |
Amino acid length |
684 |
Molecular weight |
77.2 |
Isoelectric point (calc.) |
6.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol;
SPB? |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |