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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC83.07
Gene ID 28/C08
Gene synonyms/obsolete
Gene product Lid2 complex; involved in transcriptional regulation; interacts physically with SET1 complex; involved in chromatin remodeling (regulation); zinc finger protein; jmjC domain
Entry clone Cloned
ORF length (unspliced) 2315
ORF length (spliced) 2259
Entry clone length 2315
No. of intron 1
Sequence status Finished
Sequence results 1057T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC83.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGCAAAATGTTAAAGA
Rev primer name SPBC83.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTCTAAGGAAGGAAAGAGA
Amino acid length 752
Molecular weight 84.9
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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