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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G6.05c
Gene ID 28/D03
Gene synonyms/obsolete
Gene product endosome associated protein; involved in intracellular protein transport; involved in signal transduction; BRO1-like domain
Entry clone Cloned
ORF length (unspliced) 2328
ORF length (spliced)
Entry clone length 2328
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments ApaI or SacII should be used instead of NotI for integration using pDUAL.
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17G6.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGAAACTTGCTACTCC
Rev primer name SPAC17G6.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGATGTGAAGGTTTCGCATA
Amino acid length 775
Molecular weight 86.6
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLGIYNLFL/LQALPPLPL
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
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