Gene name |
SPAC4G8.03c |
Gene ID |
28/D08 |
Gene synonyms/obsolete |
|
Gene product |
RNA-binding protein;
pumilio family; similar to Sp SPAC6G9.14 |
Entry clone |
Cloned |
ORF length (unspliced) |
2343 |
ORF length (spliced) |
|
Entry clone length |
2343 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1217C:A /
1871T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4G8.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCAATCGAGATGCTTA |
Rev primer name |
SPAC4G8.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACTGGACGTTGAGGAAGTC |
Amino acid length |
780 |
Molecular weight |
88.2 |
Isoelectric point (calc.) |
9.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LASLRNLNI/LVQHVLELNI |
Localization (YFP) |
Golgi?; a lot of
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |