Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCP25A2.03
Gene ID 28/E03
Gene synonyms/obsolete
Gene product vertebrate p84 (retinoblastoma binding) orthologue; involved in RNA processing; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 2373
ORF length (spliced) 2259
Entry clone length 2373
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCP25A2.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGGTGCAAAAGGGTTT
Rev primer name SPCP25A2.03.Rv
Rev primer SEQ AGAAAGCTGGGTATAATACAATTTCTCCATCC
Amino acid length 752
Molecular weight 85.2
Isoelectric point (calc.) 4.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKCLFAILDL/LQAILQLII
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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