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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP23A10.10
Gene ID 28/F03
Gene synonyms/obsolete
Gene product serine/threonine protein kinase
Entry clone Cloned
ORF length (unspliced) 2400
ORF length (spliced) 2250
Entry clone length 2400
No. of intron 1
Sequence status Finished
Sequence results 1087A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP23A10.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTAGCCAATGCACTTCG
Rev primer name SPBP23A10.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGAAGGCTTTTCCAGCCA
Amino acid length 749
Molecular weight 82.8
Isoelectric point (calc.) 8.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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