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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC458.05
Gene ID 28/F06
Gene synonyms/obsolete vps34
Gene product phosphatidylinositol 3-kinase; involved in stress response (implicated); class D vps; essential; involved in cell growth (required); involved in vacuole organization and biogenesis; involved in sporulation; involved in conjugation; involved in prospore membrane formation
Entry clone Cloned
ORF length (unspliced) 2406
ORF length (spliced)
Entry clone length 2406
No. of intron 0
Sequence status Finished
Sequence results 1522T:C / 2262T:G / 2340T:G / 2384T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC458.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATAGACTTGTTTTTTC
Rev primer name SPAC458.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGGATCGCATATACTGCGCA
Amino acid length 801
Molecular weight 92.1
Isoelectric point (calc.) 7.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDGLLLKLQL/LVTIPILYL/LLDFHALPL
Localization (YFP) cytoplasmic dots; cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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