Gene name |
SPBC1709.15c |
Gene ID |
28/G11 |
Gene synonyms/obsolete |
|
Gene product |
CFII component;
involved in polyadenylation |
Entry clone |
Cloned |
ORF length (unspliced) |
2457 |
ORF length (spliced) |
2394 |
Entry clone length |
2457 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1709.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTCAACTATCAATGTTT |
Rev primer name |
SPBC1709.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACGACAGCAAGTGCATCG |
Amino acid length |
797 |
Molecular weight |
89.4 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
489 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPVLNALTL |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |