| Gene name |
SPCC825.01 |
| Gene ID |
28/H04 |
| Gene synonyms/obsolete |
|
| Gene product |
ABC transporter;
unknown specificity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2469 |
| ORF length (spliced) |
|
| Entry clone length |
2469 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
570T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC825.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTAGGGGAAAGCGTTC |
| Rev primer name |
SPCC825.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTTTTTGCTTCTCCAAT |
| Amino acid length |
822 |
| Molecular weight |
92.6 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
179/190/151 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LADDMDDLSL/LTCVEAVLDI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleolus>nucleus>cytosol |
| Microscope used for
observation |
DeltaVision |