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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC16E8.07c
Gene ID 28/H06
Gene synonyms/obsolete vph1
Gene product vacuolar ATP synthase subunit; V-type ATPase subunit; vacuolar proton pump component; V0 sector
Entry clone Cloned
ORF length (unspliced) 2471
ORF length (spliced) 2418
Entry clone length 2471
No. of intron 1
Sequence status Finished
Sequence results 17A:G / 202A:G / 703A:G / 2378T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC16E8.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGGCACTTGGGGAGTT
Rev primer name SPAC16E8.07.Rv
Rev primer SEQ AGAAAGCTGGGTACTCCGCGGTCACTTTGAAA
Amino acid length 805
Molecular weight 91.2
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain 6
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LWRTLRGNLFI/LEAIFGYLVI/LICVPWLLI
Localization (YFP) ER?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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