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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC965.02
Gene ID 29/A02
Gene synonyms/obsolete SPCC1840.12
Gene product similar to oligopeptide transporter (OPT) family; similar to Sp isp4 and SPAC29B12.10C
Entry clone Cloned
ORF length (unspliced) 2490
ORF length (spliced) 2376
Entry clone length 2490
No. of intron 1
Sequence status Finished
Sequence results 1420A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC965.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAACTCCAAAATTTAT
Rev primer name SPCC965.02.Rv
Rev primer SEQ AGAAAGCTGGGTACCATCCAGAAGGTTTGCTA
Amino acid length 791
Molecular weight 89.2
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain 15
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LASIIVSLGL
Localization (YFP) cytosol=nucleus?; periphery?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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