| Gene name |
SPBC215.01 |
| Gene ID |
29/B02 |
| Gene synonyms/obsolete |
SPBC3B9.20 |
| Gene product |
TBC domain protein; EF
hand motifs; calcium binding protein; GTPase activating
protein of Rab-like GTPase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2505 |
| ORF length (spliced) |
|
| Entry clone length |
2505 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
126T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC215.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCTTTAGCCAGTCT |
| Rev primer name |
SPBC215.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCATCATTTTTTTTGTAT |
| Amino acid length |
834 |
| Molecular weight |
95 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LASLIKTKLKL/LPWFLSLFL/LRGLLDSLVI |
| Localization (YFP) |
cytosol; periphery at
site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>cytosol;
cytoplasmic dots) |
| Microscope used for
observation |
Leica |