| Gene name |
SPBC19G7.10c |
| Gene ID |
29/B10 |
| Gene synonyms/obsolete |
|
| Gene product |
topoisomerase
associated protein |
| Entry clone |
Cloned in 2004
trial |
| ORF length (unspliced) |
2520 |
| ORF length (spliced) |
2235 |
| Entry clone length |
2520 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC19G7.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCATTTTTTGGGTTTAA |
| Rev primer name |
SPBC19G7.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCGCTGAGCTGACGAGCG |
| Amino acid length |
744 |
| Molecular weight |
82.9 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LESIWKALYI/LLTIVNELDL |
| Localization (YFP) |
cytoplasmic dots;
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol;
cytoplasmic dots) |
| Microscope used for
observation |
DeltaVision |