| Gene name |
SPAC513.01c |
| Gene ID |
29/C05 |
| Gene synonyms/obsolete |
eft201; eft2; eft2-1;
SPAPYUK71.04c |
| Gene product |
elongation factor 2;
similar to Sp SPCP31B10.07 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2529 |
| ORF length (spliced) |
|
| Entry clone length |
2529 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
6C:T / 15A:T /
2524T:C |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC513.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTGCGTTCACTCCTGA |
| Rev primer name |
SPAC513.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGGCGGTCATAATATTCA |
| Amino acid length |
842 |
| Molecular weight |
93.2 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMEMIVLHL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |