| Gene name |
SPCP31B10.07 |
| Gene ID |
29/C06 |
| Gene synonyms/obsolete |
eft202 |
| Gene product |
experimentally
characterised; elongation factor 2; identical to Sp eft201
(paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2529 |
| ORF length (spliced) |
|
| Entry clone length |
2529 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
122A:G / 624T:C /
767A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCP31B10.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCGCGTTCACACCTGA |
| Rev primer name |
SPCP31B10.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAAGCGGTCATAATATTCA |
| Amino acid length |
842 |
| Molecular weight |
93.2 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMEMIVLHL |
| Localization (YFP) |
cytoplasmic dots;
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |