Gene name |
SPCC1919.12c |
Gene ID |
29/C07 |
Gene synonyms/obsolete |
|
Gene product |
protease |
Entry clone |
Cloned |
ORF length (unspliced) |
2532 |
ORF length (spliced) |
|
Entry clone length |
2532 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1919.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACAAATTCTAGACGGCA |
Rev primer name |
SPCC1919.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATATAACAATTGAAGACTTC |
Amino acid length |
843 |
Molecular weight |
95.6 |
Isoelectric point (calc.) |
7.6 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
9 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNLPIYQVLNI/LVFIVSVILSI/LVIFVVFLSL/LAVLTMLWI |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |