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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC63.08c
Gene ID 29/D03
Gene synonyms/obsolete
Gene product serine/threonine protein kinase; involved in autophagy
Entry clone Cloned in 2004 trial
ORF length (unspliced) 2555
ORF length (spliced) 2493
Entry clone length 2555
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC63.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACTTACAGACGAGTAC
Rev primer name SPCC63.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAAGATTGGGGGCAAGT
Amino acid length 930
Molecular weight 91.5
Isoelectric point (calc.) 7.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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