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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1B3.13
Gene ID 29/D06
Gene synonyms/obsolete
Gene product WD repeat protein; involved in the regulation of nucleolar silencing; involved in the regulation of the telophase exist (RENT) complex; ribonucleoprotein (RNP) complex; small subunit (SSU) processome component; involved in rRNA processing
Entry clone Cloned
ORF length (unspliced) 2574
ORF length (spliced) 2403
Entry clone length 2574
No. of intron 1
Sequence status Finished
Sequence results 1257G:A / 2204T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1B3.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGTTGGTTCATTGGA
Rev primer name SPAC1B3.13.Rv
Rev primer SEQ AGAAAGCTGGGTACTCTGTGTTCAATGTAGCT
Amino acid length 800
Molecular weight 88.3
Isoelectric point (calc.) 6.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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