Gene name |
SPAP27G11.05c |
Gene ID |
29/E04 |
Gene synonyms/obsolete |
vps41 |
Gene product |
WD repeat protein;
involved in intracellular protein transport; involved in
vacuole fusion |
Entry clone |
Cloned |
ORF length (unspliced) |
2815 |
ORF length (spliced) |
2661 |
Entry clone length |
2815 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
check |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAP27G11.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGTCGATGAGAGCAA |
Rev primer name |
SPAP27G11.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTTTGTTTATATAGCATC |
Amino acid length |
886 |
Molecular weight |
101.9 |
Isoelectric point (calc.) |
5.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMLGFNILTL/LPEIPPSLQI/LTESLAFLYL/LYLKLHSELCI/LMLIINELLDI |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |