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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC146.14c
Gene ID 29/E06
Gene synonyms/obsolete sec26; SPBC337.01c
Gene product adaptin; coatomer (beta subunit); the coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network; Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins (By similarity)
Entry clone Cloned
ORF length (unspliced) 2823
ORF length (spliced)
Entry clone length 2823
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC146.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTGTTGGACACTTGT
Rev primer name SPBC146.14.Rv
Rev primer SEQ AGAAAGCTGGGTAAACAAATGCACCCAAGCTA
Amino acid length 940
Molecular weight 104.6
Isoelectric point (calc.) 5.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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