| Gene name |
SPBC83.01 |
| Gene ID |
29/F01 |
| Gene synonyms/obsolete |
|
| Gene product |
actin cortical patch
component; predicted coiled-coil region (600-700 AA); involved
in endocytosis; EH domains; UBA domain; EF hand motifs;
calcium binding protein; similar to Sp SPBC800.10C and
SPAC25G10.09C; involved in protein deubiquitination
(implicated); ubiquitin-binding protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2850 |
| ORF length (spliced) |
2655 |
| Entry clone length |
2850 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
2092A:G /
2206T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC83.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGCCTTTGCAGTTATC |
| Rev primer name |
SPBC83.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACAATTCGTAGATTACGTGA |
| Amino acid length |
884 |
| Molecular weight |
97.8 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |