Gene name |
SPAC1006.04c |
Gene ID |
29/F06 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan; predicted coiled-coil |
Entry clone |
Cloned |
ORF length (unspliced) |
2859 |
ORF length (spliced) |
|
Entry clone length |
2859 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1669G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1006.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTAAAGAAACTCACGA |
Rev primer name |
SPAC1006.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTGTCTCCAAATGTTTGC |
Amino acid length |
952 |
Molecular weight |
109.8 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCSLVEELKL/LEKVNSELKL |
Localization (YFP) |
cytoplasmic dots;
mitochondrion |
Comments for localization |
large bright dots by
over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |