| Gene name |
SPCC162.02c |
| Gene ID |
29/G05 |
| Gene synonyms/obsolete |
|
| Gene product |
AMP-binding
dehydrogenase; involved in antibiotic synthesis in bacteria;
no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2946 |
| ORF length (spliced) |
|
| Entry clone length |
2946 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
681A:G / 881T:C /
1136A:G / 1955C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC162.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTGTGCGATTCGTCAGA |
| Rev primer name |
SPCC162.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCAATGTATAGATTCTCC |
| Amino acid length |
981 |
| Molecular weight |
109.8 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIILPNLLVL/LSRTIPKLSI/LEYLVKLNI/LISLVPSLRI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|