Gene name |
SPAC26F1.08c |
Gene ID |
29/G09 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
2977 |
ORF length (spliced) |
2934 |
Entry clone length |
2977 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
568A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC26F1.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTTCCACCATGGCTG |
Rev primer name |
SPAC26F1.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACGCCATTTTCTTCCCAATC |
Amino acid length |
977 |
Molecular weight |
111.3 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
12 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSRNLYTNLTI/LIIKLENLGI |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|