Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC922.02c
Gene ID 29/G11
Gene synonyms/obsolete SPAC1039.11c
Gene product alpha-glucosidase; glycosyl hydrolase family 3; GPI anchored protein 1; similar to Sp agl1 and SPAC30D11.01c
Entry clone Cloned
ORF length (unspliced) 2988
ORF length (spliced)
Entry clone length 2988
No. of intron 0
Sequence status Finished
Sequence results 611T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC922.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTCATCCATCGAATGAA
Rev primer name SPAC922.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGACTGGCAAATTCCAACTG
Amino acid length 995
Molecular weight 112.7
Isoelectric point (calc.) 4.5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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