| Gene name |
SPBC3H7.03c |
| Gene ID |
29/H02 |
| Gene synonyms/obsolete |
|
| Gene product |
2-oxoglutarate
dehydrogenase (lipoamide) (e1 component of oxoglutarate
dehydrogenase complex); involved in 2-oxoglutarate metabolism;
involved in tricarboxylic acid cycle; oxoglutarate
dehydrogenase (lipoamide) activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3030 |
| ORF length (spliced) |
|
| Entry clone length |
3030 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
660A:G / 1675C:T /
2490T:C / 2982G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3H7.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTCGTTTCATACCCTC |
| Rev primer name |
SPBC3H7.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGTAGGGCATCATTCAAA |
| Amino acid length |
1009 |
| Molecular weight |
114.1 |
| Isoelectric point (calc.) |
7.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAKLDPLGI |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
aggregates |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|