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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC20G8.05c
Gene ID 30/A01
Gene synonyms/obsolete cdc15
Gene product phosphoprotein; essential; involved in cytokinesis, septation,reorganization of F-actin at mitosis; similar to Sp SPBC11C11.02
Entry clone Cloned
ORF length (unspliced) 3089
ORF length (spliced) 2784
Entry clone length 3089
No. of intron 3
Sequence status Finished
Sequence results 2333G:T / 2476T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC20G8.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGGTTAATGGAGTCTC
Rev primer name SPAC20G8.05.Rv
Rev primer SEQ AGAAAGCTGGGTATACCGTCTGAACAAAGTTC
Amino acid length 927
Molecular weight 102.1
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery at cell tip and site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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