| Gene name |
SPCC1739.03 |
| Gene ID |
30/A11 |
| Gene synonyms/obsolete |
|
| Gene product |
DEAD box helicase;
involved in mRNA catabolism, nonsense-mediated |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3131 |
| ORF length (spliced) |
3048 |
| Entry clone length |
3131 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
449C:T / 611A:G /
2057T:C / 3126A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1739.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACAAGTACAAGATGA |
| Rev primer name |
SPCC1739.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTCCTTGTCAATCGCATA |
| Amino acid length |
1015 |
| Molecular weight |
116.5 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |