Gene name |
SPAC19A8.08 |
Gene ID |
30/B05 |
Gene synonyms/obsolete |
upf2 |
Gene product |
involved in the
regulation of translation |
Entry clone |
Cloned |
ORF length (unspliced) |
3150 |
ORF length (spliced) |
|
Entry clone length |
3150 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
44A:T / 2360T:C /
2579T:C / 2846A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC19A8.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAGAGAAGAACAAAT |
Rev primer name |
SPAC19A8.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCGAAGTTCAAAACCATT |
Amino acid length |
1049 |
Molecular weight |
122 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPSMVSLEL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
Microscope used for
observation |
Leica |