Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1071.02
Gene ID 30/C05
Gene synonyms/obsolete
Gene product involved in DNA repair; nucleotide-excision repair; interacts with transcription factor TFIIH
Entry clone Cloned
ORF length (unspliced) 3257
ORF length (spliced) 3057
Entry clone length 3257
No. of intron 4
Sequence status Finished
Sequence results 573A:G / 745G:A / 1519T:C / 3059A:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1071.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCTCGAATTTAGTGGC
Rev primer name SPAC1071.02.Rv
Rev primer SEQ AGAAAGCTGGGTATATATACCACTTATGACGA
Amino acid length 1018
Molecular weight 114.6
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LEYVYKCLVL/LHFIEKLNI/LAFKLLDTLNL/LPLLLQCLSL/LDTLIPSLLAI
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.