| Gene name |
SPAC29E6.10c |
| Gene ID |
30/C07 |
| Gene synonyms/obsolete |
SPAC30.14c |
| Gene product |
predicted coiled-coil;
troponin domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3258 |
| ORF length (spliced) |
|
| Entry clone length |
3258 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
43T:C / 2847A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC30.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATGATAAAGCTCTTCG |
| Rev primer name |
SPAC30.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCTGTCCAAGTACGATTT |
| Amino acid length |
1085 |
| Molecular weight |
122.9 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
716/36/703/183/705/637/704/580 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytoplasmic dots;
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |