Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC688.11
Gene ID 30/C09
Gene synonyms/obsolete
Gene product actin cortical patch component; ENTH domain protein; actin-binding protein; involved in actin cytoskeletal organization, cell polarity, endocytosis
Entry clone Cloned
ORF length (unspliced) 3279
ORF length (spliced)
Entry clone length 3279
No. of intron 0
Sequence status Finished
Sequence results 1095A:G / 1361T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC688.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGTCAGACGCATCGTT
Rev primer name SPAC688.11.Rv
Rev primer SEQ AGAAAGCTGGGTACTCTTCGGCAACATGATAA
Amino acid length 1092
Molecular weight 123.1
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLQLSKLQL
Localization (YFP) cytoplasmic dots, especially at cell tip and site of septum formation
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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