| Gene name |
SPCC1393.05 |
| Gene ID |
30/C12 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3305 |
| ORF length (spliced) |
2871 |
| Entry clone length |
3305 |
| No. of intron |
10 |
| Sequence status |
Finished |
| Sequence results |
1625G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1393.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTAAAGTATCTGCAAA |
| Rev primer name |
SPCC1393.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATAGTAGGGTGTATCCCC |
| Amino acid length |
956 |
| Molecular weight |
109 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLNFQRYLDL/LLSVECLEL |
| Localization (YFP) |
SPB; nuclear dots;
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |