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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3B9.16c
Gene ID 30/E02
Gene synonyms/obsolete
Gene product nucleoporin; nuclear pore complex
Entry clone Cloned
ORF length (unspliced) 3411
ORF length (spliced)
Entry clone length 3411
No. of intron 0
Sequence status Finished
Sequence results 872A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC3B9.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATGAATTAAAGCATGC
Rev primer name SPBC3B9.16.Rv
Rev primer SEQ AGAAAGCTGGGTATAAATCGATATTCATGGCC
Amino acid length 1136
Molecular weight 129.7
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFSLKKYLSL/LDYKLKVLDL/LDKFGALTI/LVTELVEKLFL/LIVLNTLEL
Localization (YFP) nuclear envelope
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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