| Gene name |
SPAC890.06 |
| Gene ID |
30/F01 |
| Gene synonyms/obsolete |
|
| Gene product |
nucleoporin; nuclear
pore complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4055 |
| ORF length (spliced) |
3948 |
| Entry clone length |
4055 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
98A:G / 1744A:G /
2361T:C / 3078A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC890.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACATCAATGATCCATC |
| Rev primer name |
SPAC890.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTATGTCTGACGAATTTCA |
| Amino acid length |
1315 |
| Molecular weight |
147.6 |
| Isoelectric point (calc.) |
5.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIKEVTHLLKL |
| Localization (YFP) |
cytoplasmic dots;
nuclear envelope |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |