| Gene name |
SPAC15E1.01 |
| Gene ID |
30/F12 |
| Gene synonyms/obsolete |
fat1;
SPAC15A10.16 |
| Gene product |
actin binding protein;
involved in actin cytoskeletal organization |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4158 |
| ORF length (spliced) |
|
| Entry clone length |
4158 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
2062T:C /
2884A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC15E1.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTAATAACGGCGATAA |
| Rev primer name |
SPAC15E1.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTTAGGCTTGTCTCTTCA |
| Amino acid length |
1385 |
| Molecular weight |
154.3 |
| Isoelectric point (calc.) |
4.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cell tip and site of
septum formation |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |