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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC15C4.05
Gene ID 30/G06
Gene synonyms/obsolete
Gene product ATP-dependent RNA helicase; DEAD/DEAH box helicase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 4287
ORF length (spliced)
Entry clone length 4287
No. of intron 0
Sequence status Finished
Sequence results 789A:T / 1617A:C / 1706A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC15C4.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAAAAAAAAATCAAA
Rev primer name SPBC15C4.05.Rv
Rev primer SEQ AGAAAGCTGGGTAATCTTTGAGACGTTTCTGA
Amino acid length 1428
Molecular weight 161.4
Isoelectric point (calc.) 6.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLLIHVLHL
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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