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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1919.10c
Gene ID 31/A01
Gene synonyms/obsolete myo52
Gene product class V myosin; involved in cell polarity and cytokinesis; IQ domains; DIL domain
Entry clone Cloned
ORF length (unspliced) 4684
ORF length (spliced) 4551
Entry clone length 4684
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1919.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACATCGGGGATTTATTA
Rev primer name SPCC1919.10.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTTTAATACCATTTGGT
Amino acid length 1516
Molecular weight 175.1
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots at cell tip and site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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