| Gene name |
SPBC31F10.14c |
| Gene ID |
31/A06 |
| Gene synonyms/obsolete |
|
| Gene product |
TPR repeat protein;
histone transcriptional regulator |
| Entry clone |
Cloned |
| ORF length (unspliced) |
4893 |
| ORF length (spliced) |
|
| Entry clone length |
4893 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1095A:G / 1941T:C /
2393T:G / 3987T:A / 4215T:C / 4309T:C / 4779C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC31F10.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAACGTTTACTCCTCT |
| Rev primer name |
SPBC31F10.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGAATTTCTTCATGTATA |
| Amino acid length |
1630 |
| Molecular weight |
186.7 |
| Isoelectric point (calc.) |
6.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSKLQSLLSL/LCPKIKKLQL/LHGFLDLVL/LIIHVHSLNL/LLCAFQSLKI/LCNLMALSL |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |