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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC830.01c
Gene ID 31/B02
Gene synonyms/obsolete SPCC1620.14c
Gene product SNF2 family; helicase C-terminal domain; AT hook protein; bromodomain protein; similar to Sp SPAC1250.01 (paralog)
Entry clone Cloned
ORF length (unspliced) 5043
ORF length (spliced)
Entry clone length 5043
No. of intron 0
Sequence status Finished
Sequence results 621T:C / 756A:G / 1281T:C / 4232T:C / 4932T:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC830.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTGCTGTTCAAGGGAA
Rev primer name SPCC830.01.Rv
Rev primer SEQ AGAAAGCTGGGTACTCCGAATAAGAAGAAGAA
Amino acid length 1680
Molecular weight 189.9
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 1445
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nuclear dots on spindle
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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