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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3E7.01
Gene ID 31/B10
Gene synonyms/obsolete fab1; SPBC6B1.11c
Gene product 1-phosphatidylinositol-4-phosphate 5-kinase activity; involved in signal transductionand conjugation; zinc finger protein; zf-FYVE type; induced by nitrogen starvation; non-essential
Entry clone Cloned
ORF length (unspliced) 5799
ORF length (spliced)
Entry clone length 5799
No. of intron 0
Sequence status Finished
Sequence results 1362A:G / 3109T:C / 3790G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC3E7.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAGAAGTCGAAACGCC
Rev primer name SPBC3E7.01.Rv
Rev primer SEQ AGAAAGCTGGGTATTCGCAAAACTTAAAACCT
Amino acid length 1932
Molecular weight 220.1
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVRRFEELSL
Localization (YFP) cytoplasmic dots; ambiguous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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