Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAP8A3.04c
Gene ID 31/C03
Gene synonyms/obsolete hsp9
Gene product heat shock protein
Entry clone Cloned
ORF length (unspliced) 207
ORF length (spliced)
Entry clone length 207
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAP8A3.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGATCCCGCAAGAAA
Rev primer name SPAP8A3.04.Rv
Rev primer SEQ AGAAAGCTGGGTACAACTTGTCATCAACAAAG
Amino acid length 68
Molecular weight 7.5
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.