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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC18E5.06
Gene ID 31/C08
Gene synonyms/obsolete rps21
Gene product 40S ribosomal protein S21
Entry clone Cloned
ORF length (unspliced) 317
ORF length (spliced) 264
Entry clone length 317
No. of intron 1
Sequence status Finished
Sequence results 83A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC18E5.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAAACGAGGCTGGACA
Rev primer name SPBC18E5.06.Rv
Rev primer SEQ AGAAAGCTGGGTAACGTTGATAAGACCAGACA
Amino acid length 87
Molecular weight 9.6
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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