Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19G12.06c
Gene ID 31/D04
Gene synonyms/obsolete hta2
Gene product histone h2a-beta; similar to Sp hta1
Entry clone Cloned
ORF length (unspliced) 396
ORF length (spliced)
Entry clone length 396
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC19G12.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGGAGGTAAATCTGG
Rev primer name SPAC19G12.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGCTCTTGGCTAGGCTTG
Amino acid length 131
Molecular weight 13.7
Isoelectric point (calc.) 11.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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